Alright yeast! Up ’til now, I’ve been using the lyticase for this whole spheroplasting thing for the IF, but now the gloves come OFF! No more Mrs Nice Whatsit. All of this impenetrable cell wall shit has me wicked pissed off, so I’m pouring some zymolyase on your ass.
OH YES I AM
And if that doesn’t work, then I’m going to use DTT instead of BME. And if THAT doesn’t work, I’m going to break out the glass beads. That’s right, you sons of bitches–GLASS BEADS! Because I am the multicellular, higher eukaryote here and YOU. WILL. DO. AS. I. SAY!!!
Oh, right, happy Independence Day, everyone!