Blog filler: book meme

I ran across a book meme and since my labwork has kept me too busy to write anything of substance, I thought I’d play along.  Consider yourself tagged.

1) Bold those you have read.
2) Italicize those you have started but haven’t finished.
3) Place an asterisk by those you intend to read/finish someday.

1 Pride and Prejudice – Jane Austen
2 The Lord of the Rings – JRR Tolkien
3 Jane Eyre – Charlotte Bronte
4 Harry Potter series – JK Rowling
5 To Kill a Mockingbird – Harper Lee

*6 The Bible
7 Wuthering Heights – Emily Bronte
8 Nineteen Eighty Four – George Orwell
*9 His Dark Materials – Philip Pullman
10 Great Expectations – Charles Dickens
11 Little Women – Louisa May Alcott
[One of my favorite books ever]
12 Tess of the D’Urbervilles – Thomas Hardy
13 Catch 22 – Joseph Heller
14 Complete Works of Shakespeare
15 Rebecca – Daphne Du Maurier
16 The Hobbit – JRR Tolkien
17 Birdsong – Sebastian Faulks
18 Catcher in the Rye – JD Salinger
19 The Time Traveller’s Wife – Audrey Niffenegger

20 Middlemarch – George Eliot
21 Gone With The Wind – Margaret Mitchell
*22. The Great Gatsby 0 F. Scott Fitzgerald
*23 Bleak House – Charles Dickens
24 War and Peace – Leo Tolstoy
25 The Hitch Hiker’s Guide to the Galaxy – Douglas Adams
26 Brideshead Revisited – Evelyn Waugh
*27 Crime and Punishment – Fyodor Dostoyevsky
28 Grapes of Wrath – John Steinbeck
29 Alice in Wonderland – Lewis Carroll
30 The Wind in the Willows – Kenneth Grahame
*31 Anna Karenina – Leo Tolstoy
32 David Copperfield – Charles Dickens
*33 Chronicles of Narnia – CS Lewis [I’ve read three or four but not the whole series, yet]
34 Emma – Jane Austen
35 Persuasion – Jane Austen

36 The Lion, The Witch and The Wardrobe – CS Lewis
37 The Kite Runner – Khaled Hosseini
38 Captain Corelli’s Mandolin – Louis De Bernieres
39 Memoirs of a Geisha – Arthur Golden
40 Winnie the Pooh – AA Milne

41 Animal Farm – George Orwell
42 The Da Vinci Code – Dan Brown
43 One Hundred Years of Solitude – Gabriel Garcia Marquez
44 A Prayer for Owen Meany – John Irving
45 The Woman in White – Wilkie Collins
46. Anne of Green Gables – LM Montgomery
47 Far From The Madding Crowd – Thomas Hardy
48 The Handmaid’s Tale – Margaret Atwood
49 Lord of the Flies – William Golding
50 Atonement – Ian McEwan
51 Life of Pi – Yann Martel
*52 Dune – Frank Herbert
53 Cold Comfort Farm – Stella Gibbons
54 Sense and Sensibility – Jane Austen
55 A Suitable Boy – Vikram Seth
56 The Shadow of the Wind – Carlos Ruiz Zafon
57 A Tale Of Two Cities – Charles Dickens
58 Brave New World – Aldous Huxley
59 The Curious Incident of the Dog in the Night-time – Mark Haddon
60 Love In The Time Of Cholera – Gabriel Garcia Marquez
61 Of Mice and Men – John Steinbeck
62 Lolita – Vladimir Nabokov [too damn disturbing for me to ever want to finish]
63 The Secret History – Donna Tartt
64 The Lovely Bones – Alice Sebold
65 Count of Monte Cristo – Alexandre Dumas [technically, this was an abridged version but as it was over 500 pages long, I feel it counts]
66 On The Road – Jack Kerouac
67 Jude the Obscure – Thomas Hardy
68 Bridget Jones’s Diary – Helen Fielding
69 Midnight’s Children – Salman Rushdie [absolutely brilliant]
70 Moby Dick – Herman Melville
*71 Oliver Twist – Charles Dickens
*72 Dracula – Bram Stoker
73 The Secret Garden – Frances Hodgson Burnett
74 Notes From A Small Island – Bill Bryson
75 Ulysses – James Joyce
76 The Bell Jar – Sylvia Plath
77 Swallows and Amazons – Arthur Ransome
78 Germinal – Emile Zola
*79 Vanity Fair – William Makepeace Thackeray
80 Possession – AS Byatt
81 A Christmas Carol – Charles Dickens
82 Cloud Atlas – David Mitchell
83 The Color Purple – Alice Walker
84 The Remains of the Day – Kazuo Ishiguro
85 Madame Bovary – Gustave Flaubert
86 A Fine Balance – Rohinton Mistry
87 Charlotte’s Web – EB White
88 The Five People You Meet In Heaven – Mitch Albom
*89 Adventures of Sherlock Holmes – Sir Arthur Conan Doyle
90 The Faraway Tree Collection
91 Heart of Darkness – Joseph Conrad
*92 The Little Prince – Antoine De Saint-Exupery
93 The Wasp Factory – Iain Banks
*94 Watership Down – Richard Adams
95 A Confederacy of Dunces – John Kennedy Toole
96 A Town Like Alice – Nevil Shute
97 The Three Musketeers – Alexandre Dumas
98 Hamlet – William Shakespeare
99 Charlie and the Chocolate Factory – Roald Dahl
100 Les Miserables – Victor Hugo

Two steps forward, one step back

Recently, I made some progress with the whole yeast permeablization/spheroplasting for IF problem that I have been having, but today I went back to not having any staining on my IF at all.  I know such is the nature of troubleshooting and doing science yadda, yadda, yadda, but this is really starting to get old.  On top of that, I’m not sure my committee truly wanted me to be spending this amount of time on these experiments.  True, they are the ones that suggested them, but that was when everything was working fine.  Now that it’s all gone to hell and I’ve spent weeks trying to fix it, I gotta wonder how serious they were about wanting this particular set of experiments.  Mostly because (and others in my lab agree) these experiments will not significantly add to my eventual paper (and thesis).

Advisor is on vacation.  Before he left, he said we would talk about this when he gets back.  By “talk about” I hope he means “drop these experiments and I’ll tell your committee to go fly.”

Seen on a t-shirt

“Our  ivory tower is bigger than your ivory tower”

Somehow I feel that perfectly sums up the attitude at this school.

In other news I have gotten partial permeablization of my yeast cells.  Hooray!

I think I’m going to need a trebuchet

Or perhaps just the glass beads.  The zymo did not penetrate the cell wall and I’m getting a little panicky.  I’m not sure what’s going on with these damn cells.

The damn thing is that this all started when they got rid of the warm room and I started growing the cells in incubators.  I’ve tried changing the temperature, the speed of rotation, a different incubator, all to no avail.  There is no other warm room I can try.

I am so screwed.

This. Means. WAR!

Alright yeast!  Up ’til now, I’ve been using the lyticase for this whole spheroplasting thing for the IF, but now the gloves come OFF!  No more Mrs Nice Whatsit.  All of this impenetrable cell wall shit has me wicked pissed off, so I’m pouring some zymolyase on your ass.

OH YES I AM

And if that doesn’t work, then I’m going to use DTT instead of BME.  And if THAT doesn’t work, I’m going to break out the glass beads.  That’s right, you sons of bitches–GLASS BEADS!  Because I am the multicellular, higher eukaryote here and YOU. WILL. DO. AS. I. SAY!!!

******

Oh, right, happy Independence Day, everyone!